Ezio explored the Santa Maria della Visitazione's catacombs in search of the Assassin Tomb hidden within, and after chasing down a sentry and assassinating him alongside a group of guards, Ezio entered a locked door within the catacombs.
Inside was a freerunning puzzle that involved pulling a series of levers in a certain amount of time. Doing so caused panels on a door in the center of the room to slide into place. The door then opened to reveal the tomb of the Roman Assassin Leonius.
- Within the room which contained the freerunning puzzle was an easter egg, in which a large squid would attempt to attack Ezio with one of its tentacles, with him barely dodging it.