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In 1498, Vincenzo was based in Agnadello in order to protect the Shroud, a Piece of Eden in the Order's possession. He and a group of his Brothers were sent to stop their fellow Assassin Perotto Calderon from reaching the Shroud, which Perotto intended to use for his own purposes.
Vincenzo faced off against Perotto, who attempted to simply wound and deter Vincenzo, as Perotto remembered the times when they had trained together. When Vincenzo refused to hold back, however, Perotto was forced to kill him instead.