The Vault pedestal was the central point of a First Civilization Vault and a fundamental component to its workings. Operating the chamber with which it was linked, the pedestals could often interact with a Piece of Eden, or with a descendant of the First Civilization and the human race's crossbreeding, which would therefore allow them access to whatever purpose the Vault existed for.
Santa Maria in Aracoeli
One of these pedestals could be found in the Santa Maria in Aracoeli, in Rome, and was only accessible after unlocking several levers incorporated into the structure of the building. It was used to activate an elevator that would take the pedestal's activator to the entrance of the Colosseum Vault, which was in turn opened by a hidden password. Desmond Miles found this pedestal and activated it with his DNA in 2012.
- Main article: Colosseum Vault
A second pedestal was found inside the Colosseum Vault itself. When activated, it would cause the vault to change from an almost empty chamber to being filled with glowing platforms. After completing the room's challenge, the visitor would be able to reach the central platform. In 2012, it also acted as a catalyst for Juno to take possession of Desmond Miles' body, once his DNA communed with the Apple, placed on top the pedestal.
- Main article: Pythagorean Vault
Another pedestal was located in the Pythagorean Vault, located deep inside the Temple of Pythagoras, which was visited by Ezio Auditore and Leonardo da Vinci in 1506. Leonardo discovered the temple where the Vault was located, which was rumored to contain a "perfect number" that would allow its possessor to change mankind. When Ezio placed his hand over the top of the pedestal, his DNA communed with it, filling the chamber with codes, numbers, and letters. Leonardo could not understand the meaning of it, while Ezio suggested that it was meant for someone else.