The Vaticano District (English: Vatican District) was one of the four districts of Rome. It was situated on the west bank of the Tiber river and was the location of some of Rome's greatest landmarks, such as the Basilica di San Pietro, the Cappella Sistina and the Castel Sant'Angelo.
On 28 December 1499, Pope Alexander VI gave mass in the Sistine Chapel, but was attacked by the Assassin Ezio Auditore. While the Pope survived the attack, Ezio and his uncle fled towards the Tiber after Ezio's encounter with Minerva.
- The district did not include any shops or landmarks that could be renovated.
- No Tunnels reached the district. As such, it could only be accessed by crossing the Ponte Sant'Angelo.
- It was the smallest of all districts in Italy.
- The Vatican is the only district that featured Cardinals and Papal Guards outside of memories.