The Vaticano District (English: Vatican District) was one of the four districts of Rome. It was situated on the west bank of the Tiber River and was the location of some of Rome's greatest landmarks, such as the Basilica di San Pietro, the Cappella Sistina and the Castel Sant'Angelo.
On 28 December 1499, Pope Alexander VI gave mass in the Sistine Chapel, but was attacked by the Assassin Ezio Auditore. While the Pope survived the attack, Ezio and his uncle fled towards the Tiber after Ezio's encounter with Minerva.
- The district does not include any shops or landmarks that can be renovated.
- No Tunnels reach this district; it can only be accessed by crossing the Tiber at the Centro District.
- It is the smallest of all districts in Italy.
- Upon crossing the border that separates the Vaticano district from the Centro district, the sky's color changes greatly due to the fact that the district never falls out of Borgia rule, unlike other districts in the city.
- The Vatican is the only district that features Cardinals and Papal Guards outside of memories.