The Vaticano District (English: Vatican District) was one of the four districts of Rome. It was situated on the west bank of the Tiber river and was the location of some of Rome's greatest landmarks, such as St. Peter's Basilica, the Sistine Chapel and the Castel Sant'Angelo.
On 28 December 1499, Pope Alexander VI gave mass in the Sistine Chapel, but was attacked by the Assassin Ezio Auditore da Firenze, who had crossed the Passetto di Borgo, avoiding the district. While the Pope survived the attack, Ezio and his uncle, Mario Auditore, fled towards the Tiber after Ezio's encounter with Minerva, a member of the First Civilization, in the Vatican Vault.
Later, in June of 1501, Ezio broke into the Castel Sant'Angelo and liberated Caterina Sforza, who was being held captive after the events of the siege of Monteriggioni. In the following years, he returned and located an entrance to a Lair of Romulus underneath St. Peter's.
- The district did not include any shops or landmarks that could be renovated.
- No tunnels reached the district. As such, it could only be accessed by crossing the Ponte Sant'Angelo.
- It was the smallest of all districts in Rome.
- The Vatican is the only district that featured Cardinals and Papal Guards outside of memories.