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The trailer features Ezio (and later some other Assassins) facing Cesare Borgia and his men in combat while the pope is driving through the city in his carriage. You can also notice the other Assassins wielding some new weapons, like a bow and a club, and the guard on the roof wielding a rifle.
Additionally, Assassin's Creed: Brotherhood now has a confirmed release date of November 16th 2010