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The Pope's personal chapel.
As the Pope's personal chapel, Mass is frequently held inside, as are the Papal Conclaves, which occur after the Pope's death. The cardinals are locked inside the chapel and are not permitted to leave until a new Pope is elected.
In 1492, Rodrigo Borgia became Pope Alexander VI, and moved into |St. Peter's Basilica, of which the Sistine Chapel is a part. Seven years later, in 1499, Ezio Auditore da Firenze infiltrated the chapel in order to assassinate Rodrigo Borgia and locate the Vault, which he had learned was built beneath the Basilica.
After an arduous fight with Rodrigo, in which two Pieces of Eden were used, Ezio was tricked and stabbed by Rodrigo, who had used the Papal Staff's power to immobilize the Assassin, before Ezio promptly fell unconscious.
Upon waking, Ezio pressed two hidden buttons at the back of the chapel, which opened the way into the Vault, a structure built by members of the First Civilization, whose entrance lay in a pit-like enclosure. It was at this entrance that Ezio would personally confront Rodrigo in a final, victorious fight. Soon after the Assassin left the Vault, Mario Auditore appeared and beckoned him to leave the Capella Sistina, to which Ezio complied.
- Work on Michelangelo's famous ceiling frescoes did not begin until 1508.
- Strangely, the database explicitly mentions that they "did not exist yet in 1503", despite the fact that Assassin's Creed II ends in 1499 and does not continue to 1503.