The simulation depicted a small, quaint community set near a large fortification's walls that were built into the mountainside. At the comune's center was a large open courtyard, walled in by half-open hallways, to which heralds would make various announcements. The building featured a modest bell tower as well as some stables, where a few horses rested. Residences were for the most part quite plain, with some in obvious disrepair; even the church was under construction.
San Donato, like most of the simulated locations in the first and second stage, had two aesthetic variations. As such, it could be utilized during the day or the night.
- The location had similar architecture to San Gimignano of Assassin's Creed II.
- The night variation of the location was unlocked in the first stage, once 2012 tweets were sent to the Assassin's Creed Twitter page, containing the phrase "Bring on the Night."