Roughly four years later, after Rodrigo's son, Cesare, killed him using a poison-filled apple, Cesare used the Passetto to try and reach the Apple of Eden which Rodrigo had hidden in the courtyard within St. Peter's Basilica. However, this attempt ultimately failed, as Ezio managed to race to the location first and retrieve it, shortly escaping the Vatican district afterwards.
- In Assassin's Creed II, the Passetto di Borgo was significantly larger, looking much more like a city wall than the raised walkway it is in reality.
- In Assassin's Creed II, the Passetto di Borgo was wrongly placed on the banks of the Tiber River.
- In Assassin's Creed: Brotherhood, the Passetto di Borgo was an out-of-bounds area.