Otho Holland Williams (1 March 1749 – 15 July 1794) was a Colonel in the Continental Army during the American Revolutionary War, and an ally of the Assassin Order.

In 1781, General Nathanael Greene sent Williams and several hundred men to scout the movements of Charles Cornwallis' army in North Carolina. However, Cornwallis learned of Williams' location and sent 1200 troops to attack his men. After being prompted of Williams' situation, the Colonial Assassins helped him survive the Battle of Wetzell's Mill.