Io:nhiòte was the daughter of the Assassin Ratonhnhaké:ton, granddaughter of the Templar Haytham Kenway, and great-granddaughter of the pirate Assassin Edward Kenway. The youngest of Connor's three children, she possessed the rare ability of Eagle Vision.
At some point during the 1780s, Connor married a native woman from a nearby tribe and had a son and two daughters. By April 1796, he took Io:nhiòte far from her mother's village to teach her how to track and hunt despite her tribe's tradition about training women because of his three children she was the only one with the gift. While they were hunting a deer, the young girl acted recklessly and fell from a small cliff drop, spraining her ankle. Connor left his daughter behind to look for cold water at the river in order to bring the swelling down.
While he was away, Io:nhiòte noticed fresh wolf tracks nearby and tried to warn her father of the predator's presence but he was too far away. In the sky, Connor's daughter saw an eagle above her and in her distress she somehow connected with the bird. Flying towards Connor, the Eagle screeched a warning just as the wolf was leaping on the Assassin while he was collecting water from the stream. Connor put down the wolf with his hidden blade and discovered that the wolf was driven mad by a festered wound after being shot by a musket. Returning to Io:nhiòte he was amazed to find that she was aware of his encounter with the wolf because she saw it through the eyes of an eagle. Realizing the extent of her potential, Connor stated that he had indeed a great deal to teach his daughter.
- Io:nhiòte (pronounced yo-nee-hyo-deh) means "rainbow".
- With Bayek, Io:nhiòte is one of the two known individuals gifted with Eagle Vision and who displayed the ability to telepathically see through the eyes of animals, in their case an eagle.
- The necklace she wears is in honor of her grandmother, Kaniehtí:io.