- "I have found the Achaemenid, and he gave me the secret of his poison. Not as fast as I would have hoped, but always fatal. Ironically, Alexander will die by the poison of those whom he had vanquished in battle."
- ―Iltani in a message for her Order.[src]
Iltani was a member of an ancient Order seeking to preserve free will, for which she assassinated the Macedonian king Alexander the Great via poison in 323 BCE. For her deeds and point of view, Iltani became famous amongst the Assassin Brotherhood, and is considered to be a proto-Assassin to this day.
During Iltani's lifetime, Persia was conquered by the Macedonian Empire under Alexander the Great, and she watched as the ambitious, young king swept through her homeland with a Staff of Eden provided to him by the proto-Templars.
Determined to put an end to Alexander's path of conquest, Iltani tracked him to Herat in modern-day Afghanistan, where he was holed up in a mighty citadel. Despite its high walls, she managed to infiltrate the citadel but remained apprehensive that she would be able to eliminate him through force. Sure enough, she became aware that through cunning, Alexander had somehow divined her presence within the fortress. Realizing that the risks were too great, she aborted her mission and withdrew from Herat.
Her failure did not disrupt her resolve, and she quickly devised an alternative plan, one that involved meeting with a renowned Achaemenid alchemist who was then being hunted by the Greeks. After a perilous journey, she arrived in Babylon, where she found the alchemist and acquired the secret formula for a deadly poison. While the poison was not as fast-acting as she had hoped, it was virulent enough to guarantee death.
Throughout her mission to kill Alexander, she had left messages across the lands she traveled, including India and Herat. These messages chronicled her quest and could only be deciphered through Eagle Vision. In the event that her assassination were to fail, she even left behind the recipe of the poison on her last message, hoping it would serve her Order.
This proved unnecessary, as in June 323 BCE, Iltani successfully poisoned Alexander the Great after infiltrating the palace of Nebuchadnezzar II. True to the slow nature of her poison, Alexander withered away over several days until he finally perished.
By the time of the Italian Renaissance, a tomb dedicated to Iltani was located in the Basilica di Santa Maria del Fiore in Florence. The Italian Assassin Ezio Auditore da Firenze explored her tomb and took the seal from the tomb's sarcophagus, allowing him to unlock the Armor of Altaïr Ibn-La'Ahad that was sealed in a vault under the Villa Auditore in Monteriggioni.
- Iltani is the feminine form of a Babylonian word meaning "lucky" or "favored by the god(dess)". It is also the Babylonian name for the north wind.
- Assassin's Creed II (statue only)
- Assassin's Creed: The Fall (statue only)
- Assassin's Creed Chronicles: India (mentioned only)