The Holy Grail is a mythological artifact playing an important part in Arthurian literature.
On 7 September 2012, Abstergo Industries CEO Alan Rikkin sent an email to Animus Project lead Dr. Warren Vidic that current examination of Subject Seventeen indicated that all artifacts related to Jesus Christ were literary devices or derived from Pieces of Eden, and not actual objects. Despite being unable to confirm whether the object existed and what its use might be, the company decided to focus its resources on retrieving other artifacts.
- Contrary to their previous statement, Abstergo Industries discovered during their Project Legacy initiative that the Shroud of Turin, another artifact related to Christ, is a Piece of Eden.
- A Templar legend states that the Holy Grail was found by the Order, and later taken to Scotland during the Templars' persecution in 1307, where it was buried beneath Rosslyn Chapel. Another story states that it was taken to northern Spain and protected by the Templars.
- The concept of the Chalice was introduced in Assassin's Creed: Altaïr's Chronicles. It was later revealed to be a person, Adha. This is probably a reference to the theory that the Holy Grail symbolized the bloodline of Jesus’s descendants rather than a physical object.