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Painted between 1472 and 1475, Leonardo's Annunciation was eventually purchased by Ezio Auditore da Firenze and placed in the gallery at the Villa Auditore. The painting was later lost during the Papal attack on Monteriggioni, led by the Templar Cesare Borgia.
Six years after the attack on Monteriggioni, in 1506, Ezio Auditore - whilst looking for a kidnapped Leonardo - learned that the Annunciation held part of a map identifying the location of the Temple of Pythagoras, and that the painting was in the possession of Lucrezia Borgia, who resided with her husband in Delizia di Belriguardo.
Ezio travelled to Belriguardo and, after tricking Lucrezia into revealing the painting's location, regained it for himself.