At an early age, Annetta and Paola lost their parents when they were killed at sea.
As orphans, and left with little choice of livelihood, Paola chose a life in prostitution, while Annetta became the live-in maid of the Auditore family in Florence, who were secretly a family of Assassins.
Some time after, Annetta chose to move back to Florence to stay with her sister, until her death in around 1511.
- In Assassin's Creed: Renaissance, it's mentioned that Annetta was Ezio's wet-nurse, even though she was only 2 years older than him.
- Annetta's parents supposedly died at sea in 1446 or 1447, but Annetta was born in 1457.
- Annetta is a diminutive of the name Anna, itself derived from Hebrew Channah, חַנָּה, meaning "favor" or "grace".