During the 15th century, it came into the possession of the Sultan of the Emirate of Granada, Muhammad XII, though it did not help him win his conflict against the Christian kingdoms of Iberia, Castile and Aragon. When the Spanish Inquisition under the command of Grand Inquisitor Tomás de Torquemada, a leading Templar, caught wind of Muhammad XII's treasure, they abducted his son to used as ransom for the artifact, only to be promptly deprived of it by the intervention of the Assassins Aguilar de Nerha and Maria. From Aguilar, the Apple was in turn transferred to the Italian explorer Christopher Columbus for safekeeping, and the man who would go on to expose the existence of the Americas to all of Europe would retain it until his death, bringing it to his grave.
In October 2016, the Apple was among many Pieces of Eden sought after by the Templars. They hoped that this particular Apple could provide the means to suppress humanity's violent tendencies, eliminate the free will of humanity altogether, and finally eradicate the last of the Assassin Brotherhood with the end of the Creed itself. Though Alan Rikkin, CEO of Abstergo Industries and a member of the Templar Inner Sanctum successfully recovered it from Columbus's tomb at the Seville Cathedral, he was killed by Callum Lynch, a descendant of Aguilar, while presenting it in London to the Council of Elders, whereupon possession of the Apple passed back to the Assassins.
The Apple of Eden that came into Aguilar de Nerha's possession is a small, metallic sphere encased within a protective shell that resembles amber. Unlike other Apples of Eden, this outer shell is semi-transparent when exposed to sunlight. Even with the protective shell removed, the artifact is still operational, except that onlookers can still see the Apple opening when it activates.
The first known human to possess the Apple was Muhammad XII of Granada. The Templars kidnapped the young Ahmed for the exchange between the monarch and Torquemada, his son for the Apple. Muhammad XII of Granada accepted the Templars' exchange.
Tomás de Torquemada owned the apple. Aguilar had his blade on Torquemada's throat, then he discovered that Ojeda had his own on Maria's and a standoff issued. After the assassination of Ojeda, Aguilar escaped from Torquemada and his men with the Apple. Aguilar gave the apple to Assassin ally Christopher Columbus, who took it out of the Templars' reach as he set sail for the New World. Columbus kept the Apple until his death, at which the Piece of Eden accompanied his remains back to Spain where they were buried.
In 2016, the Apple was found by the Templars after they used Callum Lynch to relive Aguilar's memories. After the Assassins broke out, they set out to recover the artifact. Alan Rikkin went to the sanctuary in London to speak to the Council of Elders, to whom he unveiled the Apple of Eden. Callum Lynch blended in with the crowd of attendants to quietly sneak up to Alan Rikkin and assassinate him with his Hidden Blade. Callum then took the Apple back and made his escape.
In chronological order:
- Muhammad XII (? – 1492)
- Tomás de Torquemada (1492)
- Aguilar de Nerha (1492)
- Christopher Columbus (1492 – 1506)
- Alan Rikkin (2016)
- Callum Lynch (2016 – ?)