Aeneas was a warrior from Troy who, according to Roman legend, led the Trojan survivors of the Trojan War on a journey to Italy, where he became a progenitor of the Roman civilization. This founding myth was propagated by the poet Virgil through his epic poem Aeneid, written during a time of sociopolitical uncertainty for the young Roman Empire. For the Romans, he served as a national hero whose tale could inspire in them vigor and hope for the future of their nation. The narrative attributes the ordeals that plagued him throughout his voyage to the bitter machinations of the goddess Juno although Juno, in reality one of the Isu, would have actually been lurking within the Grand Temple at the time as a digital consciousness.
In 2012, Aeneas was one of many historical people included in the Glyph puzzles the late Assassin Clay Kaczmarek had left behind in the Animus as messages for his successor Desmond Miles to decipher. Sometime between 8 September and 16 September, Desmond solved this puzzle, which was part of a set titled "Instruments of Power" where Aeneas was excluded from the list of historical individuals revealed by Clay to have wielded a Sword of Eden.
- Aeneas is not actually identified in the Glyph puzzle that is his only appearance in the Assassin's Creed series. His depiction is taken directly from the famous painting of Aeneas in Aeneas' Flight from Troy (1598) by Federico Barroci.
- Aeneas was also said to have been the son of Prince Anchises and the goddess of love and sexuality, Venus. In Aeneid, it is Venus who provides him with guidance along his journey, acting as the rival to Juno.